دراسة مقارنة للزرعات الذاتية والمغايرة لإصلاح الأذى المستحدث تجريبياً في الأغشية السحائية الدماغية في الكلاب

الباحث

عبدالله مزاحم أيوب عبدالله

المشرف

أسامة حازم إسماعيل الحياني

الجامعة

جامعة الموصل

الكلية / القسم

كلية الطب البيطري / الجراحة البيطرية

الشهادة

دكتوراه

السنة

2024

لغة الرسالة

انكليزي

الكلمات المفتاحية

Autograft ; Xenograft ; Meningeal Defect ; Dogs

A This project was designed to evaluate The efficiency of using different grafted tissues to reconstruct induced cerebral meningeal defects in dogs. Thirty-six local breed adult dogs were used in this experimental study. The animals were divided randomly into four main equal groups, each group consisted of nine animals. All animals were anesThetized by using a mixture of 10 % ketamine HCL at a dose of 10mg/kg and 2% xylazine at a dose of 2mg/kg respectively in The thigh muscles with The injection of atropine sulfate at a dose of 0.05mg/kg as a pre-anesThetic. A small defect was induced in The cerebral meningeal for each dog after cranirctomy. The induced menengeal defect was closed by applying different biomaterials, according to The following: First group: The meningeal defect in this group was closed by application The fascia of The temporalis muscle as autograft. Second group: The meningeal defect was closed by application apiece of peritoneum taken from The same animal as autograft also. Third group: In this group, a cellular ovine esophageal mucosa was used to close The Meningeal defect as a xenograft. Fourth group: Lyophilized bovine pericardium was used in this group as a xenograft to close The Meningeal defect. The grafted tissues in all animals were fixed on The meningeal defect using fibrin glue instead of suturing. The follow up evaluation was achieved by monitoring postoperative clinical signs for each dog especially those related with abnormal nervous signs such as hearing changes, nausea, vomiting, abnormal vision and eyeball movement, muscle spasm, or any abnormal body position. In addition, The gross and histopathological changes, estimation of cerebrospinal fluid pressure, levels of glucose, protein, and total cell count of cerebra spinal fluid in all groups at 15th, 30th, and 60th postoperative day were studied also. The statistical analysis of B histopathological scoring, cerebrospinal fluid pressure, levels of glucose, protein and total cell count of cerebrospinal fluid were dependent also. All animals after The surgical operation were survived without development of any complications especially, those related with The appearance of abnormal nervous signs, food and water intake was normally without The appearance of any change in Their behaviors. Grossly, The site of craniectomy in all animals was healed and occluded completely with connective tissue during all periods of The study externally. In addition, no cerebrospinal fluid leak was noticed at The site of craniectomy postoperatively. Also, at The end of The study, The meningeal defect was closed internally in all animals of all types of The grafted tissues used in this study sealed and connected with The meninges of The host completely. The histological study in all animals revealed by The formation of granulation tissue with infiltrations of inflammatory cells and angiogenesis. The statistical analysis of histopathological scoring was revealed a significant difference at p≤0.05 in group four than oTher groups in The degree of angiogenesis and infiltration of inflammatory cells, which characterized by more angiogenesis, and little infiltration of inflammatory cells in The early stages of healing process. In all animals, The level of protein and glucose in cerebrospinal fluid, in addition to The cerebrospinal fluid pressure appeared within The normal ranges between all groups during all periods of The study and There is no significant difference at p≤0.05. Also, The total cells count of cerebrospinal fluid was appeared within The normal range in all groups on day 15 and 60 post operation and There is no significant difference at p≤0.05 but There was a significant difference at p≤0.05 in group four on day 30 post- operation raTher than oTher groups. It could be concluded, that using of facia of temporalis muscle, peritoneum, acellular ovine esophageal mucosa, and lyophilized bovine pericardium with fibrin glue instead of suturing was successfull to close and repair cerebral meningeal defect in dogs with priority for lyophilized bovine pericardium.

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الاسم

رقم الهاتف

البريد الإلكتروني

الغرض من الطلب (اختياري)

إرسال الطلب